Journal article
Quantifying the dynamics of the oligomeric transcription factor STAT3 by pair correlation of molecular brightness
E Hinde, E Pand, Z Yang, IHW Ng, DA Jans, MA Bogoyevitch, E Gratton, K Gaus
Nature Communications | Published : 2016
DOI: 10.1038/ncomms11047
Abstract
Oligomerization of transcription factors controls their translocation into the nucleus and DNA-binding activity. Here we present a fluorescence microscopy analysis termed pCOMB (pair correlation of molecular brightness) that tracks the mobility of different oligomeric species within live cell nuclear architecture. pCOMB amplifies the signal from the brightest species present and filters the dynamics of the extracted oligomeric population based on arrival time between two locations. We use this method to demonstrate a dependence of signal transducer and activator of transcription 3 (STAT3) mobility on oligomeric state. We find that on entering the nucleus STAT3 dimers must first bind DNA to f..
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Awarded by National Institute of General Medical Sciences
Funding Acknowledgements
We thank the laboratory of Gerhard Muller-Newen for kindly providing STAT3 mutants. We thank Dr Philip Nicovich and Dr Carmine Di Rienzo for useful discussion on the analysis. E.H. is funded by the UNSW Vice Chancellor Fellowship scheme (RG124335). K.G. acknowledges funding from the ARC Centre of Excellence in Advanced Molecular Imaging (CE140100011) and National Health and Medical Research Council of Australia (1059278, 1037320). M.A.B. and D.A.J. acknowledge funding from the Australian Research Council (DP 130100804). D.A.J. acknowledges funding from the National Health and Medical Research Council Senior Principal Research Fellowship (APP1002486). E.G. acknowledges funding from the National Institute of Health (NIH P41-GM103540 and NIH P50-GM076516). The experimental work was supported by the Biomedical Imaging Facility (BMIF) at University of New South Wales.